An Alternative Method for the Detoxification and Decolorization of Industrial Waste
Noyan DOĞANGÜN, Zeynep Leyla AKIN, Alp MAZMAN
February 10, 2023 ISBN: 979-8-89480-845-1 NESEP2025
Noyan DOĞANGÜN, Zeynep Leyla AKIN, Alp MAZMAN
Abstract: To carry out detoxification and decolorization of industrial waste by using Laccase immobilized hydrogel beads which are activated by a carbodiimide, hydroxysuccininimide and carbodiimide and hydroxysuccininimide. In this paper, we have found the maximum activity % of the Laccase enzyme on hydrogel beads was observed at pH 6. Maximum activity% of the Laccase enzyme on hydrogel beads was observed at 45°C. It was observed enzyme activity and substrate concentration were directly proportional. It was observed that the enzyme activity% on the hydrogel beads maintained around 80% for
30 days for 30 days. Maximum activity% of the Laccase enzyme on hydrogel beads was observed at 80% and above for 5
usage. Laccase-immobilized hydrogel beads which are activated by a carbodiimide, hydroxysuccinimide, and both of them; can be used as an economic and alternative method for detoxification and decolorization of industrial waste. Our refining systems can be acceptable as the sustainable refining system. Our results are consistent with the results of the studies in the Literatures.
Keywords: Industrial waste, Laccase immobilized hydrogel beads, carbodiimide, enzyme activity, substrate concentration
References
- Nicell, J.A., Al-Kassim, L., Bewtra, JK., Taylor, KE., ” Wastewater treatment by enzyme catalyzed polymerization and precipitation”. Biodeterior Abstr 7: 1–8, 1993.
- Bamforth, S.M., Singleton, I., “Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions”. J Chem Technol Biotechnol 80: 723–736, 2005.
- Peppas, N.A., Ed. Hydrogels in Medicine and Pharmacy; Wiley: New York, 1987.
- Das, B., Dutta, S., Nayak, A.K., Nanda, U. (2014). Zinc alginate-carboxymethyl cashew gum microbeads for prolonged drug release: Development and optimization. International Journal of Biological Macromolecules, 70, 506–515.
- Yang, L., Shia, J., Zhoua, X., Cao, S. (2015). Hierarchically organization of biomineralized alginate beads for dualstimuli-responsive drug delivery. International Journal of Biological Macromolecules, 73, 1–8.
- https://pubchem.ncbi.nlm.nih.gov/compound/Syringaldazine
- H. Uhr; B. Mielke; O. Exner; K. R. Payne; E. Hill (2013). "Biocides". Ullmann's Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH. doi 1002/14356007.a16_563.pub2
- Dominguez, A., Rodriguez, S., Sanroman, C. and MA. “Dye decolorization by Trametes hirsuta immobilized into Alginate beads”, World Journal of Microbiology&Biotechnology 21: 405–409, 2005.